THE EFFECTS OF SOME URINARY EXTRACTS ON GASTRIC SECRETION IN THE SHAY OPERATED RAT

Abstract

Following the general procedure of Risley, Raymond, and Barnes the effects of urinary extracts on gastric secretion have been studied in the Shay operated young, male, albino rat. The procedure consisted of ligating the pylorus following a 24 hr. fast. Immediately postoperatively the test material was administered. After a five hour period the stomach was removed, opened, and rinsed into 10 ml. of distilled water. An aliquot of this was titrated for its free and total acid content. Human chorionic gonadotrophin and preparations obtained by the benzoic acid absorption method from human and equine pregnancy urine were studied for their effects on gastric section using the above technique after subcutaneous, intraduodenal, and oral administration. It was found that chorionic gonadotrophin caused gastric secretory depression at a dose level as low as 10 mgm. per rat subcutaneously. Both the human and equine preparations were observed to reduce the gastric secretion proportionately to the dose when administered subcutaneously. When administered by intraduodenal injection at the time of operation, the equine preparation was active but required several times the subcutaneous dose to produce comparable results. Oral administration of both equine and human pregnancy urine extracts showed antisecretory activity only in the former at the dose levels employed. In addition, the effects of possible complicating factors such as estrogens, pyrogens, and nonspecific damage on gastric secretion have been investigated and found to play no appreciable part in the results produced by the urine fractions.