Cloning, characterization, and chromosomal localization of human liver form cytochrome c oxidase subunit VIa related genes

Abstract

The chromosomal location of human cytochrome c oxidase (COX) subunit VIa Liver (VIa-L) isoform related sequences has been determined by a combination of in situ hybridization and analysis of human–hamster somatic cell hybrid panels. COX VIa-L related sequences were present on chromosomes 6 and 12. It has been verified that at least two COX VIa-L genes are on chromosome 6, one of which is a pseudogene. In total, four COX VIa-L related sequences have been cloned and their nucleotide sequences analyzed. At least three of these sequences represent pseudogenes; their relatedness to the COX VIa-L cDNA is discussed.Key words: human, cytochrome c oxidase, chromosomal localization, COX VIa, cloning.