Author:
Bajpai Urmi,Kuhad R C,Khanna Sunil
Abstract
Uptake of octadecane by Acinetobacter calcoaceticus S19 was found to be 70% during growth (doubling time of 4 h), of which 60% was incorporated into the cells and 40% was oxidized to 14CO2. The ratio of [14C]octadecane uptake to its mineralization by whole cells was similar to that found during the growth of A. calcoaceticus S19. After 4 h of incubation of cells with [14C]octadecane, 44% was mineralized to 14CO2, while the rest remained associated with the cells. Octadecane uptake was not observed under anaerobic conditions, indicating an absolute requirement for oxygen. Acinetobacter calcoaceticus S19 converted octadecane to the corresponding octadecanol and octadecanoic acid; the corresponding aldehyde was not detected, however. Octadecanoic acid was partially degraded through β-oxidation to CO2 and partly assimilated as cell biomass.Key words: Acinetobacter calcoaceticus, octadecane uptake, mineralization.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
8 articles.
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