Mutualistic degradation of the lignin model compound veratrylglycerol-β-(o-methoxyphenyl) ether by bacteria

Abstract

Complete degradation of the lignin model compound veratrylglycerol-β-(o-methoxyphenyl) ether is accomplished mutualistically by a two-membered bacterial culture. Bacterial isolate E1, which has been tentatively identified as an Acinetobacter, grows on veratrylglycerol-β-(o-methoxyphenyl) ether producing guaiacol (o-methoxyphenol) as a non-metabolizable, bacteriocidal by-product. When Nocardia corallina (strain A81) is also present in media containing veratrylglycerol-β-(o-methoxyphenyl) ether as the only carbon/energy source, it is able to grow on the guaiacol produced from veratrylglycerol-β-(o-methoxyphenyl)ether by isolate E1. Strain A81 alone does not grow on veratrylglycerol-β-(o-methoxyphenyl) ether. In the absence of strain A81, isolate E1 is rapidly killed by accumulated guaiacol. In the presence of the Nocardia, isolate E1 maintains its viability.