Use of phage JHJ-1 to assess restriction in the genus Saccharopolyspora

Abstract

Restriction of phage growth occurred when phage JHJ-1 propagated in one member of the genus Saccharopolyspora (S. hirsuta, S. taberi, S. rectivirgula, or the erythromycin-producing S. erythraea) was used to infect a different member. Of several S. erythraea strains tested, S. erythraea NRRL 2359 exhibited the weakest restriction. Phage SE-3 did not appear to be strongly restricted in Saccharopolyspora strains, whereas restriction of phages FRb-P and FRa-C was confined mainly to S. hirsuta NRRL B-5792. There was no correlation between the restriction of phage growth and the presence of exocytoplasmic DNAse. Key words: Saccharopolyspora, restriction, host range, bacteriophage JHJ-1, bacteriophage SE-3, bacteriophage FRa-C, bacteriophage FRb-P.