Author:
Chyi Y.-S.,Hoenecke M. E.,Sernyk J. L.
Abstract
A genetic linkage map has been constructed based on restriction fragment length polymorphism DNA markers for Brassica rapa L. using a segregating F2 progeny from a cross between the yellow sarson type 'R500' and the canola-type 'Horizon'. The map contains 360 marker loci detected by 269 genomic clones derived from a PstI library of 'Westar' (Brassica napus L.). The map consists of 10 linkage groups, covering a total of 1876 recombination units. The occurrence of substantial genome rearrangement during the evolution of B. rapa is evident from (i) a large number of homologous duplicate sequences either at genetically adjacent locations or in different linkage groups and (ii) a number of missing sequences in one or the other parental genome, detected as null alleles. Divergence of the parental genotypes is further supported by the frequency of loci with skewed allele segregation ratios. Comparison of molecular and cytogenetic data on genome structure is discussed. The probe set and map are being used to facilitate rapeseed breeding.Key words: Brassica campestris, Brassica rapa, genome structure, linkage map, restriction fragment length polymorphism.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,General Medicine,Biotechnology
Cited by
106 articles.
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