Functional and structural successions in arbitrary samples of heterotrophic bacteria during aerobic treatments of lignite-carbonization wastewater inin situenclosures

Abstract

In situ mesocosm experiments were performed in Lake Schwelvollert (located in the district of Weißenfels, Saxony-Anhalt, Germany), an anaerobic lignite-carbonization effluent lake containing phenolic compounds and their autoxidation products (anthropogenic humic matter). In the aeration enclosure, the anaerobic Schwelvollert wastewater was aerated and in the flocculation enclosure, it was flocculated to precipitate the oxygen-trapping anthropogenic humic matter to enhance the input of oxygen by diffusion. To gain an insight into the metabolic state of the aerobic heterotrophic microbiota during the treatments, arbitrary samples of bacterial isolates were taken from a general agar medium and tested for their abilities to cleave predominant phenolic contaminants by a procedure called the isolate sample assay. In this way, successions of degradation potentials were observed in both mesocosms, with degradation abilities for meta- and para-alkylated phenols appearing before degradation abilities for ortho-substituted phenols as a common phenomenon. To examine the structure of samples, the respective isolates were characterized using the Biolog GN MicroPlate system, the random amplified polymorphic DNA nucleic acid (RAPD) fingerprinting technique, and amplified ribosomal DNA restriction analysis (ARDRA). Although similar functional patterns occurred in both mesocosms, the compositions and diversities of the respective bacterial communities varied significantly, even at different depths from the same enclosure, with members of the Pseudomonas RNA group I being predominant.Key words: lignite carbonization, phenols, mesocosm, isolate sample assay, Biolog, RAPD, ARDRA.