A CHEMICALLY DEFINED MEDIUM FOR GROWTH OF PASTEURELLA MULTOCIDA

Abstract

The chemically defined medium of McKenzie et al. was modified by increasing dextrose and buffer and adding metallic salts. This modified medium supported growth of nine different isolates of P. multocida through 10 serial transfers. One isolate (strain X-73) was successfully subcultured through more than 100 transfers in the modified medium without change of its biochemical characteristics. Viable organisms were recovered from static cultures after 45 days" incubation at 37 C. A 1/10-ml inoculum containing approximately two or three organisms was sufficient to produce good growth within 24–48 h.