Vesicle self-assembly of amphiphilic siderophores produced by bacterial isolates from Soap Lake, Washington

Abstract

Soap Lake, located in Washington State, is a meromictic soda lake that was the subject of a prior National Science Foundation funded Microbial Observatory. Several organisms inhabiting this lake have been identified as producers of siderophores that are unique in structure. Two isolates found to be of the species Halomonas, SL01 and SL28, were found to produce suites of amphiphilic siderophores consisting of a peptidic head-group, which binds iron appended to fatty acid moieties of various lengths. The ability for siderophores to self-assemble into vesicles was determined for three suites of amphiphilic siderophores of unique structure (two from SL01 and one from SL28). These siderophores resemble the amphiphilic aquachelin siderophores produced by Halomonas aquamarina strain DS40M3, a marine bacterium. Vesicle self-assembly studies were performed by dynamic light scattering and epifluorescence microscopy. The addition of ferric iron (Fe3+) at different equivalents, where an equivalence of iron is defined as equal to the molarity of the siderophore, demonstrated vesicle formation. This was suggested by both dynamic light scattering and epifluorescence microscopy. Bacteria thriving under saline and alkaline conditions are capable of producing unique siderophores that self-assemble in micelles and vesicles due to ferric iron chelation.