A rapid gas-extraction technique for the quantitative study of denitrification in aquatic systems by N-isotope ratio analysis

Author:

Chan Y. K.,Campbell N. E. R.

Abstract

A rapid technique was developed for the extraction and preparation of gas samples required for field studies of aquatic denitrification. With this method, the natural atom percent abundance of 15N in air-saturated distilled water was found to be 0.370 ± 0.004 SD. Nitrogen-15 tracer experiments showed that 20% of the nitrate taken up was denitrified in 5 days in lake water samples collected after fall turnover. Under conditions conducive to denitrification, 90% of the nitrate taken up was denitrified in 2 days by pure cultures of a Pseudomonas species.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Forest detritus and cycling of nitrogen in a mountain lake;Canadian Journal of Forest Research;1991-07-01

2. ASSAYS OF MICROBIAL NITROGEN TRANSFORMATIONS;Nitrogen in the Marine Environment;1983

3. Denitrification in Lake 227 During Summer Stratification;Canadian Journal of Fisheries and Aquatic Sciences;1980-03-01

4. Measurement of Denitrification in Two Freshwater Sediments by an In Situ Acetylene Inhibition Method;Applied and Environmental Microbiology;1979-06

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