Construction and characterization of the first bacterial artificial chromosome library for the cotton species Gossypium barbadense L.-Reference-Cited by-同舟云学术

Construction and characterization of the first bacterial artificial chromosome library for the cotton species Gossypium barbadense L.

Published:2006-11 Issue:11 Volume:49 Page:1393-1398
ISSN:0831-2796
Container-title:Genome
language:en
Short-container-title:Genome

Author:

Wang X.F.¹²,Ma J.¹²,Wang W.S.¹²,Zheng Y.M.¹²,Zhang G.Y.¹²,Liu C.J.¹²,Ma Z.Y.¹²

Affiliation:

1. Key Laboratory of Crop Germplasm Resources of Hebei Province, Agricultural University of Hebei, Baoding, 071001, China

2. CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, Brisbane, QLD, 4067, Australia

Abstract

As the second most widely cultivated cotton, Gossypium barbadense is well known for its superior fiber properties and its high levels of resistance to Fusarium and Verticillium wilts. To enhance our ability to exploit these properties in breeding programs, we constructed the first bacterial artificial chromosome (BAC) library for this species. The library contains 167 424 clones (49 920 BamHI and 117 504 HindIII clones), with an estimated average insert size of 130 kb. About 94.0% of the clones had inserts over 100 kb, and the empty clones accounted for less than 4.0%. Contamination of the library with chloroplast clones was very low (0.2%). Screening the library with locus-specific probes showed that BAC clones represent 6.5-fold genome equivalents. This high-quality library provides an additional asset with which to exploit genetic variation for cotton improvement.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,General Medicine,Biotechnology

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/g06-113

Reference27 articles.

1. Targeted isolation of simple sequence repeat markers through the use of bacterial artificial chromosomes

2. A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene

3. Restriction fragment length polymorphisms in diploid and allotetraploid Gossypium: Assigning the late embryogenesis-abundant (Lea) alloalleles in G. hirsutum

4. Genetic Effects of ThirteenGossypium barbadenseL. Chromosome Substitution Lines in Topcrosses with Upland Cotton Cultivars: I. Yield and Yield Components

5. QTL Analysis of Cotton Fiber Quality Using Multiple Gossypium hirsutum × Gossypium barbadense Backcross Generations

Cited by 16 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Transcriptome, Ectopic Expression and Genetic Population Analysis Identify Candidate Genes for Fiber Quality Improvement in Cotton;International Journal of Molecular Sciences;2023-05-05

2. Evolution, expression and functional analysis of cultivated allotetraploid cotton DIR genes;BMC Plant Biology;2021-02-10

3. Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum;PLOS ONE;2018-05-17

4. Genetic Mapping in Cotton;Past, Present and Future Trends in Cotton Breeding;2018-05-02

5. Genome Sequencing;Agronomy Monographs;2015-10-28