The recalcification plasma clotting time. A valuable general clotting test in man and rats

Abstract

An improved method for determining the recalcification plasma clotting time (PCT) in siliconized and plastic material is described. Provided the plasma is stored at 29–30 °C, this general clotting test appears to give highly accurate and reproducible results. The PCT values were found to be related to the in vivo platelet count in rat, and to the total serum cholesterol and plasma fibrinogen in man. Serial determinations in two women indicated that the PCT was subject to periodic fluctuations connected with the menstrual cycle. Finally, it was observed that the clotting time of both platelet-rich and -poor plasma was inversely related to the severity of large hepatic vein thrombosis initiated by the injection of a S. typhosa endotoxin in hyperlipemic rats.