Isocitrate lyase in Coprinus lagopus (sensu Buller)

Abstract

Isocitrate lyase activity was detected in cell-free extracts of both a monokaryon and a dikaryon of Coprinus lagopus sensu Buller grown in media containing either acetate or glucose as carbon source. The activity in extracts of mycelium grown in an acetate medium was greater than that in extracts of mycelium grown in a glucose medium but marked differences between extracts of monokaryons and dikaryons grown in similar media were not observed. The isocitrate lyase activity could be largely sedimented by centrifugation at 10 000 × g for 20 minutes.