Invitro plant regeneration via callus culture of mature Salixexigua

Abstract

Callus induction, callus growth, and plantlet regeneration were examined using leaf explants of three Salixexigua clones. Calli were initiated on three basal media supplemented with 0.1 mg/L (0.44 μM) or 0.5 mg/L (2.2 μM) of benzylaminopurine and 0.1 mg/L (0.45 μM) or 0.5 mg/L (2.3 μM) of 2,4-dichlorophenoxyacetic acid in a factorial fashion. After 7 weeks of growth, callus production was highest on woody plant medium. Developed calli were subsequently cultured on woody plant medium supplemented with either 0.1 or 0.5 mg/L of benzylaminopurine for shoot induction. Shoot primordia developed only at 0.1 mg/L (0.44 μM) benzylaminopurine in two clones, suggesting clonal variation in organogenic response. Shoots larger than 1.0 cm in length were successfully rooted in half-strength Murashige and Skoog medium without hormones.