THE ACTIVATION OF GLUTAMIC ACID AND GLUTAMINE IN MAMMALIAN TISSUES

Author:

Alford M. A.,Brotman M.,Chudy M. A.,Fraser M. J.

Abstract

Measurements of α-glutamyl-RNA synthetase and glutaminyl-RNA synthetase activities have been made in fractions derived from 105,000 × g supernatants of homogenates of rat liver and of mouse Ehrlich ascites carcinoma cells. Evidence is presented which indicates that each amino acid is activated by a specific enzyme. Both enzymes catalyze amino acid dependent ATP-32PP exchange. The α-glutamyl-RNA synthetase purified 42-fold from rat liver 105,000 × g supernatant is free of glutamine synthetase.

Publisher

Canadian Science Publishing

Subject

General Medicine

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Control of cell-free protein synthesis by amino acids: Effects on tRNA charging;International Journal of Biochemistry;1985-01

2. Complex of aminoacyl-transfer RNA synthetases;Journal of Molecular Biology;1971-08

3. Rat Liver Glutamyl Ribonucleic Acid Synthetase;Journal of Biological Chemistry;1967-03

4. Glutamyl and Glutaminyl Ribonucleic Acid Synthetases of Escherichia coli W;Journal of Biological Chemistry;1965-01

5. PARTIAL PURIFICATION AND PROPERTIES OF PROLYL-RNA SYNTHETASE OF RAT LIVER;Canadian Journal of Biochemistry and Physiology;1963-10-01

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