Author:
Bouchard M.,Chaudhary K. D.,Loiselle J.-M.,Lemonde A.
Abstract
The acid-soluble phosphate compounds in the pupal extract of Tribolium confusum Duval have been fractionated quantitatively on an AG1-X4 resin column using a concave gradient of LiCl and HCl. Nine principal components corresponding to catechol, xanthosine, uric acid, AMP, ADP, UDP, CTP, ATP, and UTP have been characterized by their elution patterns, chromatographic properties on Ecteola-cellulose, and ultraviolet absorption spectra. The possible significance of relatively higher concentrations of catechol and uric acid has been discussed. Xanthine probably represents an intermediary stage in the uric acid synthesis. Adenine nucleotides occur in higher concentrations than uracil and cytosine nucleotides, A particularly higher level of ATP seems to suggest an accumulation of this compound as a result of low energy requirements during the pupal stage, which, being a non-growth phase, manifests lower synthetic activity.
Publisher
Canadian Science Publishing