Slow troponin C is present in both muscle and nonmuscle cells

Abstract

A common primer was used for synthesis of cDNAs from both chicken fast and slow troponin C (TnC) mRNAs. Synthesis of double-stranded cDNAs and their amplification by polymerase chain reaction gave specific products corresponding to these mRNAs. This method was used for determining the presence of TnC mRNAs in various tissues. Our results show that while the fast TnC mRNA is expressed only in the muscle cells, slow TnC mRNA is expressed in a number of nonmuscle cells. Not all nonmuscle cells, however, express slow TnC mRNA. Liver and brain tissues showed the presence of high levels of this mRNA, while it was absent in chicken smooth muscle and embryonic skin. The slow TnC mRNA was very stable in cardiac muscle cells. It degrades with a half-life of approximately 94 h. The same mRNA was less stable in skeletal muscle and liver cells. The half-lives were found to be only between 13 and 16 h in these cells. Our results suggest that slow TnC mRNA may function as the nonmuscle isoform of this contractile protein. Since slow TnC mRNA is the only TnC isoform present in cardiac muscle, liver and brain, it is possible that besides its role in regulating contraction of striated muscle slow TnC can also function in processes other than muscle contraction.Key words: troponin C mRNA, cardiac muscle, skeletal muscle, polymerase chain reaction, nonmuscle troponin C.