Author:
Broad T. E.,Lewis P. E.,Pearce P. D.,Phua S. H.,Forrest J. W.,Pugh P. A.,Stewart-Scott I. A.
Abstract
A DNA repeat element, revealed initially by digestion of horse DNA with TaqI, was cloned and characterized by Southern and in situ hybridization studies and nucleotide sequencing. The clone, e4/1, consisted of 32 tandem reiterations of a unit repeat of 21–22 bp, and produced multilocus DNA fingerprinting profiles that were useful for parentage analysis in horses. The tandem repeat element was shown by in situ hybridization to be localized in the centromeres of the acrocentric but not metacentric classes of horse chromosomes.Key words: Equidae, Equus caballus, DNA repeat, DNA fingerprinting, centromere.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,General Medicine,Biotechnology
Cited by
9 articles.
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