Author:
Dufresne M. J.,Igarashi S. J.
Abstract
A procedure for the preparation of a large quantity of biologically active, highly purified ribosomes from rabbit liver is described. The method employs polyethylene glycol – dextran sulfate partition and DEAE-cellulose chromatography to overcome the limitations encountered in conventional procedures. The entire process takes only 48 h to obtain 10 000 A260 units of ribosomes. The ribosomes thus obtained are predominantly 78 S particles with a constant protein–RNA ratio of 0.95. The ribosomes are free from RNase, amino-acyl-tRNA synthetase, and amino-acyl-tRNA:protein transferase activity. The protein synthesizing activity is dependent on added mRNA and protein factors. These ribosomes are stable for prolonged periods of storage in a liquid nitrogen refrigerator.
Publisher
Canadian Science Publishing
Cited by
2 articles.
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