"Portraying" of plant genomes using polymerase chain reaction amplification of ribosomal 5S genes

Abstract

Nontranscribed spacers of plant genes coding for ribosomal 5S RNA were amplified using the polymerase chain reaction. Primers were synthesized that were complementary to 3′ (direct) and 5′ (reverse) ends of the coding region and that are universal for higher plants. The patterns of polymerase chain reaction products are species and, sometimes, variety specific. The use of this approach for identification of barley 5S genes in chromosome-addition lines of wheat is discussed. This principle can be applied for the "portraying" of other tandem repetitive genes containing divergent regions.Key words: polymerase chain reaction, 5S genes, plants, DNA polymorphism.