Use of sepharose-conjugated BCG antibodies for the purification of tuberculin-active components

Abstract

Bacillus Calmette–Guérin (BCG) antibodies conjugated with CNBr-activated Sepharose 4B led to the isolation of tuberculin-active components from the protoplasmic materials of BCG. From these results, it is concluded that most of the tuberculin-active components (sensitins)have mycobacterial antigenic properties.