Heteroplasmy and paternally oriented shift of the organellar DNA composition in barley–wheat hybrids during backcrosses with wheat parents

Abstract

Mitochondrial (mt) and chloroplast (ct) genome inheritance was studied in barley–wheat hybrids, as were their progenies obtained from backcrosses with different common wheat cultivars, by monitoring the composition of 4 mtDNA (coxI, a 5′-flanking region of cob, nad3-orf156, and 5′-upstream region of 18S/5S) and 2 ctDNA (simple-sequence repeat locus downstream of trnS and a 3′-flanking region of rbcL) loci. In male sterile F1and BC1plants, maternal barley mtDNA fragments were mainly detected and very low levels of paternal wheat fragments were occasionally detected by PCR in coxI, a 5′-flanking region of cob and nad3-orf156, whereas a 5′-upstream region of 18S/5S showed clear heteroplasmy, containing both maternal and paternal copies, with maternal copies prevailing. Plants showing such heteroplasmic mtDNA composition remained either semisterile or became completely sterile in the later backcross generations. Only maternal ctDNA copies were detected in these plants. In 3 stable, self-fertile, and vigourous lines obtained in the advanced backcross generations and possessing recombinant wheat nuclear genome, however, only mt- and ctDNA copies of wheat parents were detected; thus, the original alloplasmic condition appeared to be lost. Our results suggest that transmission followed by selective replication of the paternal wheat organellar DNA leads to a paternally oriented shift of the organellar DNA composition in barley–wheat hybrids, which correlates with the restoration of fertility and plant vigour. These 2 processes seem to be related to nucleocytoplasmic compatibility and to be under the control of the nuclear genome composition.Key words: barley–wheat hybrids, ctDNA, mtDNA, nucleoytoplasmic compatibility, organelle DNA inheritance.