Numerous chloroplast DNA polymorphisms are shared among different populations and species in the aureoid Senecio (Packera) complex

Abstract

Species boundaries within the aureoid Senecio complex (Packera) are poorly defined, possibly as a result of hybridization and introgression. The results of restriction site analysis of cpDNA using 16 enzymes and hybridization with lettuce probes representing over 95% of the chloroplast genome reveal a high level of intrapopulational polymorphism combined with a relatively low level of interspecific variation. Forty-nine populations representing 23 different species were examined, and only 20 variable restriction sites were identified. Eleven of the 20 variable sites were polymorphic in at least one population, and 14 of the 23 species were polymorphic for at least one site. The polymorphisms are widespread and are found in species representing all of the previously recognized subgroups of the aureoid complex. Individuals analyzed separately were shown to possess only a single haplorype. Although the polymorphisms may be the result of hybridization and introgression, it is difficult to explain the geographical patterns based on the current distribution of species. It is possible that the aureoid complex represents a single, polymorphic biological species. Keywords: Asteraceae, Senecio, aureoid complex, Packera, cpDNA, molecular systematics.