Author:
Führer L.,Kubicek C. P.,Röhr M.
Abstract
A reliable method for the extraction and assay of pyridine nucleotides (NAD, NADH, NADP, and NADPH) in filamentous fungi is presented. The method is applied to a study of the physiology of citric acid accumulation by Aspergillus niger. The measurement of "catabolic reduction charge" (NADH/(NAD + NADH)) and "anabolic reduction charge" (NADPH/(NADP + NADPH)) as a means of characterizing the metabolic state is discussed.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
34 articles.
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