Abstract
A relatively simple and reliable in vitro methodology for fish chromosome study is described. A single cell suspension obtained by trypsinizing ovarian tissue was cultured for 5–7 days at 31 C. The cells were treated with colchicine and hypotonic solutions then fixed in acetic-alcohol fixative. Air-dried preparations were made from these cells and were stained with aceto-orcein.Numerous metaphase plates unmarred by overlapping chromosomes were recovered and high quality karyograms were available for detailed karyotypic analysis. The general similarity of squash and air-dried karyotypes suggests that short-period cell culture preserves natural karyotypes.
Publisher
Canadian Science Publishing
Cited by
30 articles.
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