Interspecific variation in heat tolerance and evaporative cooling capacity among sympatric temperate-latitude bats

Abstract

We tested the hypothesis that interspecific variation in chiropteran heat tolerance and evaporative cooling capacity is correlated with day-roost microclimates, using three vespertilionid bats that occur sympatrically during summer in Saskatchewan, Canada. We predicted that hoary bats (Lasiurus cinereus (Palisot de Beauvois, 1796); ∼22 g) would have higher heat tolerance than little brown bats (Myotis lucifugus (Le Conte, 1831); ∼7 g) and silver-haired bats (Lasionycteris noctivagans (Le Conte, 1831); ∼13 g), as the latter two species roost in tree crevices or cavities that are more thermally buffered than the foliage roosts of hoary bats. We measured core body temperature (Tb; passive integrated transponder tags), evaporative water loss, and resting metabolic rate (flow-through respirometry) while exposing individuals to a stepped profile of increasing air temperature (Ta) from ∼30 °C in ∼2 °C increments. Experiments were terminated when individuals became hyperthermic (Tb ≈ 42.5 °C), with maximum Ta (Ta,max) ranging from 42.0 to 49.7 °C. As predicted, hoary bats had the highest heat tolerance and evaporative cooling capacity, reaching Ta,max ∼2.4 and 1.2 °C higher than little brown and silver-haired bats, respectively. Our results are consistent with the hypothesis that heat tolerance of bats is correlated with roost microclimates, although interspecific variation in body mass and phylogeny may confound these conclusions.