CHROMATOGRAPHY OF COLIPHAGES T1 AND T2 ON COLUMNS OF CALCIUM PHOSPHATE

Abstract

Coliphages T1 and T2 were efficiently adsorbed from dialyzed lysates by small columns of calcium phosphate. Elution of the columns with phosphate buffers at low molarity (0.02–0.03) removed much of the non-infectious material; the phage could subsequently be eluted in more concentrated and purified form with higher buffer concentrations (0.06 M or greater). The chromatographic behavior of T1 and T2 is contrasted and the influence of non-phage constituents of the lysate upon the chromatographic separation is discussed.