Patterns of Incorporation of [3H]leucine and [32P]orthophosphate into Nonhistone Proteins of Rainbow Trout during Early Development

Abstract

In rainbow trout eggs developing at 10 °C, the relative amounts of nonhistone proteins of the embryo nuclei change during the course of cleavage; the relative amounts of at least two of the proteins seem to increase whereas four decrease during this period. [4,5-3H]leucine is incorporated into all components that stain visibly with Coomassie blue from mid-blastula on, and into approximately seven components at early blastula. [32P]orthophosphate is incorporated into three of the components of the nonhistone proteins, which also stain intensely with Coomassie blue. The results support a role for the nonhistone proteins in the control of differentiation and are in sharp contrast to the lack of change in the relative concentrations of the histones following the late blastula stage.