Author:
Altman Eleonora,Brisson Jean-Robert,Messner Paul,Sleytr Uwe B.
Abstract
The cell surface of the mesophilic eubacterium Bacillus alvei CCM 2051 is covered by an oblique arranged surface layer glycoprotein. The subunits revealed by sodium dodecyl sulfate – polyacrylamide gel electrophoresis were distinct bands of molecular masses 140 000, 128 000, and 127 000. Proteolytic degradation of the purified S-layer glycoprotein yielded a single glycopeptide fraction with an apparent molecular mass of ca. 25 000. Methylation analysis in conjunction with two-dimensional nuclear magnetic resonance experiments at 500 MHz established the branched trisaccharide[Formula: see text]as the repeating unit for this glycan chain.Key words: surface layer, eubacteria, glycoprotein, nuclear magnetic resonance, structure.
Publisher
Canadian Science Publishing
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
37 articles.
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