Investigating membrane protein dynamics in living cellsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.-Reference-Cited by-同舟云学术

Investigating membrane protein dynamics in living cellsThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.

Published:2006-12 Issue:6 Volume:84 Page:825-831
ISSN:0829-8211
Container-title:Biochemistry and Cell Biology
language:en
Short-container-title:Biochem. Cell Biol.

Author:

Bates Ian R.¹²,Wiseman Paul W.¹²,Hanrahan John W.¹²

Affiliation:

1. Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montréal, QC H3G 1Y6, Canada

2. Department of Chemistry, McGill University, Montréal, QC H3A 2K6, Canada.

Abstract

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

Publisher

Canadian Science Publishing

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

http://www.nrcresearchpress.com/doi/pdf/10.1139/o06-189

Reference24 articles.

1. Fluorescence cross-correlation spectroscopy in living cells

2. Probing the Endocytic Pathway in Live Cells Using Dual-Color Fluorescence Cross-Correlation Analysis

3. A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy

4. Membrane Lateral Diffusion and Capture of CFTR within Transient Confinement Zones

5. Quantitative analysis of the formation and diffusion of A1-adenosine receptor-antagonist complexes in single living cells

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