Action of ethylenediaminetetraacetic acid, tris(hydroxymethyl)aminomethane, and lysozyme on cell walls of Pseudomonas aeruginosa

Author:

Cox Jr. S. T.,Eagon R. G.

Abstract

Incubation of isolated cell walls of Pseudomonas aeruginosa with tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), and lysozyme, either singly or in combinations, results in partial solubilization of the cell walls.When cell walls were incubated with a combination of Tris and EDTA, approximately 30% of the total cell wall and 32% of the lipopolysaccharide fraction were solubilized. The following percentages of the individual cell wall constituents were solubilized: protein, 19%; carbohydrate, 35%; lipid, 5%; ash, 54%; and, phosphorus 23%. When lysozyme was included with Tris and EDTA, approximately 36% of the total cell wall and 86% of the lipopolysaccharide fraction were solubilized. Solubilization of the individual cell wall constituents was similarly increased.Phospholipids, which make up 7–8% of the intact cell wall of P. aeruginosa, were not released by incubation of the cell walls with these agents. Fatty acids in addition to those found in the lipopolysaccharide, however, were detected in the solubilized cell wall materials.Incubation systems composed of Tris, of EDTA, and of Tris and lysozyme solubilized cell walls to a lesser extent than incubation systems of Tris and EDTA, and of Tris, EDTA, and lysozyme. Incubation of cell walls in water, furthermore, was effective in solubilizing approximately 8% of the cell walls. Muramic acid containing materials were solubilized only in the presence of lysozyme.Materials liberated from cell walls which were incubated in systems containing EDTA exhibited single symmetrical Schlieren peaks characterized by uncorrected sedimentation constants of approximately 7 S, suggesting the release of homogenous subunits from the cell walls.Intra- and inter-molecular cross-linking by divalent cations via phosphate groups contained in lipoprotein and lipopolysaccharide components of the cell wall of P. aeruginosa is proposed.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

Cited by 43 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3