EVIDENCE OF A MICROSOMAL FACTOR REQUIRED FOR IN VITRO DEVELOPMENT OF PSEUDO-TRYPTOPHAN SYNTHASE ACTIVITY BY EXTRACTS OF NEUROSPORA CRASSA

Abstract

Extracts of conidia of the td3mutant strain of Neurospora crassa did not develop pseudo-tryptophan synthase enzyme activity under conditions leading to formation of the activity by equivalent extracts of the wild type strain. The defect in extracts of the td3mutant appears to be located in the microsome fraction. The latter is unable to interact effectively with a component of the td3mutant "post-microsome" fraction which will support in vitro development of the enzymic activity when supplemented with microsomes from other strains of N. crassa.