Sponge phylogeny, animal monophyly, and the origin of the nervous system: 18S rRNA evidence

Abstract

We sequenced 18S rRNA genes of a calcareous sponge, Clathrina cerebrum, a demosponge, Axinella polypoides, and a zoanthid cnidarian, Parazoanthus axinellae. Our phylogenetic analysis supports the monophyly of kingdom Animalia and confirms that choanoflagellate protozoans are their closest relatives. Sponges as a whole are monophyletic, but possibly paraphyletic; demosponges and hexactinellids form a monophyletic group of silicious sponges. Our phylogenetic trees support a monophyletic origin of the nervous system in the immediate common ancestor of Cnidaria and Ctenophora. They weakly suggest that animals with a nervous system may be more closely related to calcareous sponges than to silicious sponges; the nervous system might have originated in an early calcareous sponge. Our trees confirm that Myxozoa and Placozoa are animals that arose by secondary loss of the nervous system, but suggest that Myxozoa may be sisters of, rather than derived from, Bilateria. Kingdom Animalia is divided into four subkingdoms: Radiata (Porifera, Cnidaria, Placozoa, Ctenophora), Myxozoa, Mesozoa, and Bilateria. The 18S rRNA genes of Myxozoa evolved over twice as fast as in Radiata. Comparison with the fossil record reveals a brief 10-fold (or greater) acceleration in the rate of rRNA evolution in early Bilateria followed by normal low rates for about 500 million years.