Exigences en sels minéraux de Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh.

Abstract

The mineral requirements of Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh., were studied in a medium containing seven amino acids, three vitamins, dextrose, and tris buffer. The organism does not grow in a mineral-free medium. Potassium phosphate monobasic and potassium phosphate dibasic are required in concentrations of 1.2 × 10−3 and 2.8 × 10−3 mole per liter respectively. Ammonium phosphate monobasic inhibits the growth. Potassium can be replaced by calcium when H3PO4 is used as a source of PO4---. Magnesium as MgCl2∙6H2O in concentration of 2 × 10−3 mole per liter is required for good growth but does not affect the morphology of the organism as reported for other bacteria. Manganese as MnSO4>∙H2O and iron as Fe(SO4)2(NH4)2∙6H2O, in concentrations of 10−3 and 10−6 mole per liter respectively, are not essential but stimulate the growth when added to potassium phosphate (KH2PO4 and K2HPO4) and magnesium. Copper, nickel, cobalt, aluminum, and zinc were found to be inhibitory.