Suitability of some enrichment broths and diluents for enumerating cold- and heat-stressed Vibrio parahaemolyticus

Abstract

Vibrio parahaemolyticus cells were injured by chilling and heating, and their recovery was tested in glucose–salt–Teepol broth (GSTB), tryptic soy broth containing 7% NaCl (TSBS), Horie – arabinose – ethyl violet broth (HAEB), and water blue – alizarin yellow broth (WBAY). Exponential phase cells were more sensitive to cold shock than were stationary phase cells. Exposure of chill-injured V. parahaemolyticus to GSTB and TSBS resulted in 70 to 80% death; about 70% lethality was noted for heat-injured cells inoculated into TSBS. Neither HAEB nor WBAY enrichment media were lethal to stressed cells, although rates of growth were retarded. The 3% NaCl in 0.1 M potassium phosphate (pH 7.0) diluent proved to be most suitable for protecting against inactivation of cold- and heat-injured cells.