Cryopreservation of zebra fish spermatozoa using methanol

Abstract

We describe a method for cryopreservation of milt from individual Brachydanio rerio using methanol and powdered milk as cryoprotectants. Motility was positively correlated with hatching, which averaged 51 ± 35.6% in a typical experiment. Variablity in motility and hatching was not correlated with sperm volume or age of the fish, and is believed to be due to differences in sperm quality between individuals, as well as technical constraints imposed by the short duration of motility in thawed spermatozoa.