THE INTERACTIVE EFFECT OF ULTRAVIOLET IRRADIATION AND 5-BROMOURACIL AT THE RIB-1 LOCUS IN NEUROSPORA CRASSA

Abstract

5-Bromouracil (5-BU) was shown to enhance reverse mutation rates when added to conidia of a rib-1 strain of Neurospora crassa previously irradiated with ultraviolet light. A comparison is presented between various incubation intervals in the presence and absence of 5-BU while varying the UV dose. An increase in mutation frequency ranging from 0.028–2.05 × 102 times greater than the spontaneous frequency, UV frequency alone or 5-BU frequency alone was observed when UV irradiation and 5-BU were combined.