The Decay of Xenopus laevis Mitochondrial rRNA in the Presence of Ethidium Bromide or Actinomycin D
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Published:1974-10-01
Issue:10
Volume:52
Page:911-915
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ISSN:0008-4018
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Container-title:Canadian Journal of Biochemistry
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language:en
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Short-container-title:Can. J. Biochem.
Author:
Young P. G.,Zimmerman A. M.
Abstract
The effect of ethidium bromide and actinomycin D on prelabelled mitochondrial RNA was studied in Xenopus laevis embryos. Mitochondrial rRNA was found to decay with a half-life of 2–2.5 h following an initial lag of < 2 h. There was an initial increase in incorporation into mitochondrial rRNA in the presence of actinomycin D but not in the presence of ethidium bromide. Radioactivity in mitochondrial 4 S RNA rises and then is relatively stable for the duration of the chase period. Cytoplasmic rRNA and 4 S RNA are stable under the chase conditions used, and, in the presence of ethidium bromide, cytoplasmic RNA continues to be synthesized.
Publisher
Canadian Science Publishing