Developmental neurotoxic effects of a low dose of TCE on a 3-D neurosphere system

Author:

Abdraboh M.E.1,Abdeen S.H.1,Salama M.2,El-Husseiny M.2,El-Sherbini Y.M.3,Eldeen N.M.1

Affiliation:

1. Department of Zoology, Faculty of Science, Mansoura University, Mansoura 35116, Egypt.

2. Department of Toxicology, Faculty of Medicine, Mansoura University, Mansoura 35116, Egypt.

3. Faculty of Health and Life Science, Oxford Brookes University, Headington Campus, Oxford, OX3 0BP, UK.

Abstract

Trichloroethylene (TCE) is one of the industrial toxic byproducts that now persist in the air, soil, and water. Several studies have already illustrated the toxic effect of high doses of TCE on the biological functions of several organs. This study aims to highlight the toxic impact of a low dose of TCE (1 μmol/L) on the development of rat neural stem cells (NSCs). The subventricular zones (SVZ) of rat pup’s brains were collected and minced, and the harvested cells were cultured in the presence of neural growth factors B27/N2 to develop neurospheres. The cells were then exposed to a dose of 1 μmol/L TCE for 1 or 2 weeks. The outcomes indicated a remarkable inhibitory effect of TCE on the differentiation capacity of NSCs, which was confirmed by down-regulation of the astrocyte marker GFAP The inhibitory effect of TCE on the proliferation of NSCs was identified by the reductions in neurosphere diameter, Ki67 expression, and cell cycle arrest at the G1/S phase. Immunolabelling with annexin V indicated the proapoptotic effect of TCE exposure. PCR results revealed a TCE-mediated suppression of the expression of the antioxidant enzyme SOD1. This paper illustrates, for the first time, a detailed examination of the toxic effects of an environmentally low dose of TCE on NCSs at the transcriptional, translational, and functional levels.

Publisher

Canadian Science Publishing

Subject

Cell Biology,Molecular Biology,Biochemistry

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