Mechanisms of carbon acquisition for endosymbiont photosynthesis in Anthozoa

Abstract

In contrast to free-living photoautotrophs, endosymbiontic dinoflagellates of the genus Symbiodinium must absorb their inorganic carbon from the cytoplasm of their host anthozoan cell rather then from seawater. The purpose of this paper is to review the present knowledge on the source of dissolved inorganic carbon supply for endosymbiont photosynthesis and the transport mechanisms involved. Symbiodinium spp., generally known as zooxanthellae, live within the endodermal cells of their hosts, corals and sea anemones. They are separated from the surrounding seawater by the host tissues (oral ectodermal cell layer, collagenous basal membrane, endodermal cell, and perisymbiotic vesicles). The symbiotic association is therefore faced with the problem of delivering dissolved inorganic carbon to an endodermal site of consumption from an, essentially, ectodermal site of availability. Studies using original methods demonstrated that neither the internal medium (coelenteric fluid) nor paracellular diffusion could supply enough dissolved inorganic carbon for endosymbiont photosynthesis. A transepithelial active mechanism must be present in the host tissues to maintain the photosynthetic rate under saturating irradiance. A pharmacological approach led to propose a working model of dissolved inorganic carbon transport from seawater to zooxanthellae. This vectorial transport generates a pH gradient across the epithelium. The role of this gradient as well as the physiological adaptation of Symbiodinium spp. to symbiotic life are discussed.Key words: carbon concentrating mechanism, anthozoan, dinoflagellates, anion transport, symbiosis, transepithelial transport.