A one-megabase physical map provides insights on gene organization in the enormous mitochondrial genome of cucumber

Author:

Bartoszewski Grzegorz1234,Gawronski Piotr1234,Szklarczyk Marek1234,Verbakel Henk1234,Havey Michael J.1234

Affiliation:

1. Department of Plant Genetics, Breeding, and Biotechnology, Faculty of Horticulture and Landscape Architecture, Warsaw University of the Life Sciences, ul. Nowoursynowska 159, 02-776 Warsaw, Poland.

2. Department of Genetics, Plant Breeding, and Seed Production, Agricultural University of Krakow, al. 29 Listopada 54, 31-425 Krakow, Poland.

3. Keygene N.V, Agro Business Park 90, 6708 PW Wageningen, The Netherlands.

4. US Department of Agriculture, Department of Horticulture, Agricultural Research Service, Vegetable Crops Unit, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706, USA.

Abstract

Cucumber ( Cucumis sativus ) has one of the largest mitochondrial genomes known among all eukaryotes, due in part to the accumulation of short 20 to 60 bp repetitive DNA motifs. Recombination among these repetitive DNAs produces rearrangements affecting organization and expression of mitochondrial genes. To more efficiently identify rearrangements in the cucumber mitochondrial DNA, we built two nonoverlapping 800 and 220 kb BAC contigs and assigned major mitochondrial genes to these BACs. Polymorphism carried on the largest BAC contig was used to confirm paternal transmission. Mitochondrial genes were distributed across BACs and physically distant, although occasional clustering was observed. Introns in the nad1, nad4, and nad7 genes were larger than those reported in other plants, due in part to accumulation of short repetitive DNAs and indicating that increased intron sizes contributed to mitochondrial genome expansion in cucumber. Mitochondrial genes atp6 and atp9 are physically close to each other and cotranscribed. These physical contigs will be useful for eventual sequencing of the cucumber mitochondrial DNA, which can be exploited to more efficiently screen for unique rearrangements affecting mitochondrial gene expression.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,General Medicine,Biotechnology

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