THE DEGRADATION OF DESOXYRIBONUCLEIC ACID DURING ALKALINE HYDROLYSIS
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Published:1956-03-01
Issue:2
Volume:34
Page:265-269
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ISSN:0576-5544
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Container-title:Canadian Journal of Biochemistry and Physiology
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language:en
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Short-container-title:Can. J. Biochem. Physiol.
Abstract
The extent of alkaline degradation of desoxyribonucleic acid (DNA) was determined by following the formation of organic phosphate soluble in 10% trichloroacetic acid solution and the liberation of ammonia. Hydrolysis of DNA with 1 N sodium hydroxide at 100 °C. for four hours was required to effect complete formation of acid-soluble oligonucleotides and the ammonia liberated was equivalent to the estimated amino nitrogen content of the DNA.
Publisher
Canadian Science Publishing