TRISOME IDENTIFICATION IN SORGHUM BICOLOR (L.) MOENCH BY OBSERVING PROGENY OF TRIPLOID × TRANSLOCATION STOCKS

Abstract

Crosses between triploids (3n) × translocation-tester-stocks (2n) in Sorghum bicolor (L.) Moench produced F1 trisomics heterozygous for an interchange. These trisomics were used to associate the trisomes with the translocated chromosomes. This method was used because four of the trisomics of sorghum are not sufficiently fertile to make recovery of trisomic F1 progeny possible from trisomic × translocation-tester-stock crosses.Over 91% of the progeny from the triploid × translocation-tester-stock crosses were F1 plants and 41.6% were F1 primary trisomics. Frequency of the 10 trisomic types varied from 1.9 to 7.1% of the total.The 10 trisomes of sorghum were identified cytologically to be chromosome A-bottle brush, B-sparse branch, E-compact, D-stiff branch, E-large glume, F-asymmetric, G-cone, H-light green, I-small glume and J-spear.