Cultivation of Bradyrhizobium CB756 on sucrose prefermented by yeast

Abstract

Saccharomyces cerevisiae, grown with sucrose as a substrate, converts the sucrose to glycerol and produces yeast cells. The yield of glycerol was doubled by adding CaCO3. The fermented medium, containing glycerol and yeast cells, was an excellent medium for the cultivation of Bradyrhizobium CB756. Best results were obtained after dilution of the fermented medium to 10% (v/v) with a basal salts solution, but even with dilutions of 5 and 2.5%, high numbers of Bradyrhizobium CB756 cells were obtained. Key words: Bradyrhizobium, glycerol, nitrogen fixation, inoculants.