Abstract
The nonionic detergent Lubrol WX showed diverse, concentration-dependent effects on basal and stimulated adenylate cyclases. Above concentrations of 0.001–0.01% Lubrol WX, the basal activity of cyclase from Ehrlich ascites cells was inhibited about 50%, and that from rat fat cells was doubled. In both cases, hormonal sensitivity was lost at 0.01%. These effects were reversed upon dilution of the detergent. It is suggested that solubilization of adenylate cyclases at such low concentrations of Lubrol should be attempted since it is conceivable that loss of hormone sensitivity may then be reversible. Different Lubrol-type detergents may also offer certain advantages, since Lubrol PX effects were not identical with those of Lubrol WX.
Publisher
Canadian Science Publishing
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献