Immunofluorescence of Aeromonas hydrophila as measured by fluorescence photometric microscopy

Abstract

Strain-specific fluorescent antibodies were prepared for three isolates of Aeromonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared antisera which indicated that other undetected serogroups are present. Statistical analyses indicated that the A. hydrophila isolated from aquatic habitats were distinct from the other isolates as measured by immunofluorescence.