Analysis of Genetic Diversity in 73 Kentucky Bluegrass Materials by SSR and SRAP Markers

Abstract

Kentucky bluegrass (Poa pratensisL.) (KBG) is a commonly used grass that possesses excellent quality, as well as a complex genetic background and reproductive patterns. In this study, a total of 73 KBG germplasms were collected, of which 49 were imported varieties, 5 were Chinese breeding varieties, and 19 were wild materials. A total of 70 simple sequence repeat (SSR) and 75 sequence-related amplification polymorphism (SRAP) markers were selected to use for genetic diversity analysis. From these studies, high levels of polymorphisms were observed in SRAPs (91.8%) and SSRs (94.5%), respectively. Three dendrograms that were generated from SRAP, SSR, and SRAP+SSR combined data revealed a general similarity for the positioning of the majority of materials. However, certain materials, including Z65, Z25, and Z27, were found to be located in diverse clusters among different dendrograms. Further analysis demonstrated no significant association between geographical origin and molecular marker clusters in the wild materials. Combined with the seedling phenotype identification carried out in our prior study, it seems as though there is no significant relationship between agronomic characterization and marker-based clustering in these materials, except for in the case of leaf color. These studies provided an increased understanding of genetic diversity among KBG materials, which will be beneficial for genetic improvement and germplasm conservation in the future.