Effect of Gamma Radiation on Protein Profile, Protein Fraction and Solubility’s of Three Oil Seeds: Soybean, Peanut and Sesame

Abstract

The proteins properties of three oil seeds (soybean, peanut and sesame) were investigated following γ-irradiation (0.0, 0.5, 1.0, 2.0, 3.0, 5.0 and 7.5 KGy). The effect of γ-radiation on total protein solubility, albumin, globulin and SDS-ME fractions were studied using SDS-polyacrylamide gel electrophoresis. The results showed that solubility of total protein were decreased and reached to the maximum decrease using irradiation dose of 7.5 KGy compared to control. The interesting phenomena are that albumin and globulin fractions decreased in its solubility while the SDS-ME fraction increased. These phenomena may be due to the effect of gamma radiation on the protein, which may dissociate this fraction to small subunits, and rearrangement to form a complex protein even high or small molecular weight proteins solubilized only in SDS-ME fraction. The changes in protein profile were depended even on radiation dose and on the nature of oil seeds; soybean, peanut and sesame.