Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases

Author:

SUNAGA Fujiko1,TSUCHIAKA Shinobu23,KISHIMOTO Mai2,AOKI Hiroshi4,KAKINOKI Mari1,KURE Katsumasa5,OKUMURA Hanako5,OKUMURA Maho6,OKUMURA Atsushi7,NAGAI Makoto12,OMATSU Tsutomu23,MIZUTANI Tetsuya23

Affiliation:

1. Laboratory of Infectious Disease, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan

2. Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo 183–8509, Japan

3. The United Graduate School of Veterinary Sciences, Gifu University, Yanagito, Gifu 501-1193, Japan

4. Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino-shi, Tokyo 180-8602, Japan

5. Value Farm Consulting Co., Ltd., 1704-3 Nishi Oi, Tsukuba, Ibaraki 300-1260, Japan

6. Drexel University Dornsife School of Public Health, Philadelphia PA 19104, USA

7. Centre for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA

Publisher

Japanese Society of Veterinary Science

Subject

General Veterinary

Reference29 articles.

1. 1. Angen, O., Jensen, J. and Lavritsen, D. T. 2001. Evaluation of 5′ nuclease assay for detection of Actinobacillus pleuropneumoniae. J. Clin. Microbiol. 39: 260–265.

2. 2. Bonifait, L., Veillette, M., Létourneau, V., Grenier, D. and Duchaine, C.Detection of streptococcus suis in bioaerosols of swine confinement buildings.Appl. Environ. Microbiol. 80: 3296–3304.

3. 3. Edington, N., Wrathall, A. E. and Done, J. T. 1988. Porcine cytomegalovirus (PCMV) in early gestation. Vet. Microbiol. 17: 117–128.

4. 4. Giammarioli, M., Pellegrini, C., Casciari, C. and De Mia, G. M. 2008. Development of a novel hot-start multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus. Vet. Res. Commun. 32: 255–262.

5. 5. Grau-Roma, L. and Segalés, J. 2007. Detection of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine influenza virus and Aujeszky’s disease virus in cases of porcine proliferative and necrotizing pneumonia (PNP) in Spain. Vet. Microbiol. 119: 144–151.

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