Stable expression of red fluorescent protein-blasticidin deaminase fusion gene (<i>rfp-bsd</i>) as a selectable marker for DNA transfection in <i>Babesia ovata</i>
Author:
Affiliation:
1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
Funder
Japan Society for the Promotion of Science
Japan-Czech Republic Research Cooperative Program between JSPS and CAS
Publisher
Japanese Society of Veterinary Science
Link
https://www.jstage.jst.go.jp/article/jvms/86/7/86_24-0111/_pdf
Reference13 articles.
1. 1. Asada M, Goto Y, Yahata K, Yokoyama N, Kawai S, Inoue N, Kaneko O, Kawazu S. 2012. Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. PLoS One 7: e35227.
2. 2. Asada M, Yahata K, Hakimi H, Yokoyama N, Igarashi I, Kaneko O, Suarez CE, Kawazu S. 2015. Transfection of Babesia bovis by double selection with WR99210 and blasticidin-S and its application for functional analysis of thioredoxin peroxidase-1. PLoS One 10: e0125993.
3. 3. Brayton KA, Lau AO, Herndon DR, Hannick L, Kappmeyer LS, Berens SJ, Bidwell SL, Brown WC, Crabtree J, Fadrosh D, Feldblum T, Forberger HA, Haas BJ, Howell JM, Khouri H, Koo H, Mann DJ, Norimine J, Paulsen IT, Radune D, Ren Q, Smith RK Jr, Suarez CE, White O, Wortman JR, Knowles DP Jr, McElwain TF, Nene VM. 2007. Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoa. PLoS Pathog 3: 1401–1413.
4. 4. Cubillos EFG, Snebergerova P, Borsodi S, Reichensdorferova D, Levytska V, Asada M, Sojka D, Jalovecka M. 2023. Establishment of a stable transfection and gene targeting system in Babesia divergens. Front Cell Infect Microbiol 13: 1278041.
5. 5. Cuesta I, González LM, Estrada K, Grande R, Zaballos A, Lobo CA, Barrera J, Sanchez-Flores A, Montero E. 2014. High-quality draft genome sequence of Babesia divergens, the etiological agent of cattle and human babesiosis. Genome Announc 2: e01194–e14.
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